Targeted P2X7R shRNA delivery attenuates sympathetic nerve sprouting and ameliorates cardiac dysfunction in rats with myocardial infarction
发布时间：2019-05-13 分享到：
…(ii)sense:5′‐CUCUUGAGGAGCGCCGAAA‐3′; antisense: 5′‐UUUCGGCGCUCCUCAAGAG‐3′. The double‐strand oligonucleotides were ligated into the RNAi‐Ready pSIREN‐DNR‐DsRed‐Express Vector (Biomiao, Beijing, China). Then, the donor vector was inserted into the pLP‐Adeno‐X LP CMV Vector (Biomiao) to be constructed as the recombinant pLP‐Adeno‐X P2X7R shRNA (Ad‐sh P2X7R5a)….
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