Association of ENAM, TUFT1, MMP13, IL1B, IL10 and IL1RN gene polymorphism and dental caries susceptibility in Chinese children
发布时间：2019-05-10 分享到：
…Non-irritating saliva (2 ml) was collected from each child and added to saliva protection fluid (Olegene saliva DNA collector; Guangzhou Deep Blue Gene Technology, Guangzhou, China). The saliva samples were stored at –20C until processing. Genomic DNA was extracted from each saliva sample using a Genomic DNA Isolation Kit (BioMiao Biological Technology, Beijing, China) according to the manufacturer’s instructions. All of the seven SNPs in the six candidate genes were genotyped using iPLEX MassARRAYVR system (Agena Bioscience, San Diego, CA, USA), which was similar to that used in previous research.16…..
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