A functional copy-number variation in MAPKAPK2 predicts risk and prognosis of lung cancer
发布时间:2019-05-10 分享到:
… We genotyped the g.CNV-30450 for all 4,789 subjects by using the TaqMan real-time quantitative PCR (qPCR) method according to the protocol of Applied BioSystems (catalog no. Hs01173160, Applied BioSystems, Foster City, CA, USA)11 and validated the results with the AccuCopy assay (a multiple competitive real-time PCR) by Genesky Bio-Tech (Shanghai, China) and Affymetrix Genome-Wide Human SNP Array 6.0 by Bio Miao Biological Technology (Beijing) in 200 randomly selected samples (Figures S1 and S2);12 the results were 97.5% or 98.0% concordant…
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