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Combination therapy with TNFR-Fc and CTLA4-FasL using the recombinant adeno-associated virus potently
F Wang, J Yu, Y Wang, Y Jiang, N Guo - Applied Microbiology and Biotechnology - 24 February 2015
… the standards. The recombinant AAV2.TNFR-Fc (AAV.TRFC) vector was obtained from Biomiao Biological Technology Company (Beijing, China), and the viral titer was 3 × 10 12 vector genomes/ml (vg/ml). The recombinant … -
Increased Levels of the Long Intergenic Non–Protein Coding RNA POU3F3 Promote DNA methylation in es
WeiLi JianZheng JieqiongDeng YongheYou - Gastroenterology - June 2014
… The mass spectra were collected using a MassARRAY Compact MALDI-TOF (Sequenom; BioMiaoBiological Technology, Beijing, China) and the spectra's methylation ratios were generated by theEpiTYPER software (Sequenom, San Diego, CA). Cell Viability Assay … -
Promoter methylation of fas apoptotic inhibitory molecule 2 gene is associated with obesity and dysli
Lijun Wu, Xiaoyuan Zhao, Yue Shen, Mei-Xian Zhang, Yinkun Yan, Dongqing Hou - Diabetes and Vascular Disease Research - February 12, 2015
The Sequenom MassARRAY platform (BioMiao Biological Technology, Beijing, China) was used to quantitatively analyse the methylation of FAIM2 [NC_000012.12 (49866896 … 49903977)]. The target DNA regions were amplified via polymerase chain reaction (PCR) using bisulphite-modified DNA. Specific primers are listed in Supplementary Table 1. -
A microRNA-135a/b binding polymorphism in CD133 confers decreased risk and favorable prognosis of l
Mei Cheng Lei Yang Rongrong Yang Xiaorong Yang Jieqiong Deng - Carcinogenesis - 28 May 2013
MassArray (Sequenom, San Diego, CA) was used for genotyping the 13 SNPs in the discovery set using allele-specific matrix-assisted laser desorption–time of flight mass spectrometry by commercial company (Biomiao Biological Technology, Beijing, China). Then the promising SNPs were further genotyped in the validation sets with MassArray (Sequenom). -
Genetic variability of ERCC1 and ERCC2 influences treatment outcomes in gastric cancer
W.H. Yu , Y.X. Wang , J.Q. Guo, Y.L. Wang , J.S. Zheng and K.X. Zhu - Genetics and Molecular Research - December 21, 2015
… The design of the assay and single nucleotide polymorphism genotyping were performed by Bio Miao Biological Technology (Beijing, China) using the Sequenom MassARRAY platform (Sequenom, San Diego, CA, USA) according to the manufacturer instructions … -
Association between single nucleotide polymorphisms in NFATC1 signaling pathway genes and susceptibil
Fengyu WangHaili WangLina WangShiyuan Zhou - Pediatric Cardiology - 27 August 2016
… the standard protocol. The primers (Table 1) were designed by MassARRAY Assay Design 3.1 Software (Sequenom) and synthetized by Biomiao Biological Technology Company (Beijing, China). Sequencing was performed … -
Cochleovestibular gene transfer in neonatal mice by canalostomy
Guo, Jing-Ying; Liu, Yu-Ying; Qu, Teng-Fei; Peng, Zhe; Xie, Jing; - NeuroReport - 2 August 2017
… Materials and methods AAV8 vector A purified AAV8 vector with the green fluorescence protein (GFP) gene (AAV8-GFP), driven by the cytomegalovirus promoter, was purchased from BioMiao Biological Technology Co. Ltd (Beijing, China) … -
Lung-specific RNA interference of coupling factor 6, a novel peptide, attenuates pulmonary arterial h
Jie Yin, Shuling You, Nannan Li, Shouhai Jiao, Hesheng Hu, Mei Xue - Respiratory Research - 4 August 2016
… CF6 shRNA was designed based on the siRNA sequence (GenBank Acc. NM_053602.2) using an siRNA design tool (Biomiao, Beijing, China) and was screened according to the guidelines reported by Elbashir SM et al. [12] …
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-
Combination therapy with TNFR-Fc and CTLA4-FasL using the recombinant adeno-associated virus potently
F Wang, J Yu, Y Wang, Y Jiang, N Guo - Applied Microbiology and Biotechnology - 24 February 2015
… the standards. The recombinant AAV2.TNFR-Fc (AAV.TRFC) vector was obtained from Biomiao Biological Technology Company (Beijing, China), and the viral titer was 3 × 10 12 vector genomes/ml (vg/ml). The recombinant … -
Increased Levels of the Long Intergenic Non–Protein Coding RNA POU3F3 Promote DNA methylation in es
WeiLi JianZheng JieqiongDeng YongheYou - Gastroenterology - June 2014
… The mass spectra were collected using a MassARRAY Compact MALDI-TOF (Sequenom; BioMiaoBiological Technology, Beijing, China) and the spectra's methylation ratios were generated by theEpiTYPER software (Sequenom, San Diego, CA). Cell Viability Assay … -
Promoter methylation of fas apoptotic inhibitory molecule 2 gene is associated with obesity and dysli
Lijun Wu, Xiaoyuan Zhao, Yue Shen, Mei-Xian Zhang, Yinkun Yan, Dongqing Hou - Diabetes and Vascular Disease Research - February 12, 2015
The Sequenom MassARRAY platform (BioMiao Biological Technology, Beijing, China) was used to quantitatively analyse the methylation of FAIM2 [NC_000012.12 (49866896 … 49903977)]. The target DNA regions were amplified via polymerase chain reaction (PCR) using bisulphite-modified DNA. Specific primers are listed in Supplementary Table 1. -
A microRNA-135a/b binding polymorphism in CD133 confers decreased risk and favorable prognosis of l
Mei Cheng Lei Yang Rongrong Yang Xiaorong Yang Jieqiong Deng - Carcinogenesis - 28 May 2013
MassArray (Sequenom, San Diego, CA) was used for genotyping the 13 SNPs in the discovery set using allele-specific matrix-assisted laser desorption–time of flight mass spectrometry by commercial company (Biomiao Biological Technology, Beijing, China). Then the promising SNPs were further genotyped in the validation sets with MassArray (Sequenom). -
Genetic variability of ERCC1 and ERCC2 influences treatment outcomes in gastric cancer
W.H. Yu , Y.X. Wang , J.Q. Guo, Y.L. Wang , J.S. Zheng and K.X. Zhu - Genetics and Molecular Research - December 21, 2015
… The design of the assay and single nucleotide polymorphism genotyping were performed by Bio Miao Biological Technology (Beijing, China) using the Sequenom MassARRAY platform (Sequenom, San Diego, CA, USA) according to the manufacturer instructions … -
Association between single nucleotide polymorphisms in NFATC1 signaling pathway genes and susceptibil
Fengyu WangHaili WangLina WangShiyuan Zhou - Pediatric Cardiology - 27 August 2016
… the standard protocol. The primers (Table 1) were designed by MassARRAY Assay Design 3.1 Software (Sequenom) and synthetized by Biomiao Biological Technology Company (Beijing, China). Sequencing was performed … -
Cochleovestibular gene transfer in neonatal mice by canalostomy
Guo, Jing-Ying; Liu, Yu-Ying; Qu, Teng-Fei; Peng, Zhe; Xie, Jing; - NeuroReport - 2 August 2017
… Materials and methods AAV8 vector A purified AAV8 vector with the green fluorescence protein (GFP) gene (AAV8-GFP), driven by the cytomegalovirus promoter, was purchased from BioMiao Biological Technology Co. Ltd (Beijing, China) … -
Lung-specific RNA interference of coupling factor 6, a novel peptide, attenuates pulmonary arterial h
Jie Yin, Shuling You, Nannan Li, Shouhai Jiao, Hesheng Hu, Mei Xue - Respiratory Research - 4 August 2016
… CF6 shRNA was designed based on the siRNA sequence (GenBank Acc. NM_053602.2) using an siRNA design tool (Biomiao, Beijing, China) and was screened according to the guidelines reported by Elbashir SM et al. [12] …