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Role of IL-17 gene polymorphisms in the susceptibility to gastric cancer
W.T. Qi , J.L. Gao and S.S. Zhang1 - Genetics and Molecular Research - October 26, 2015
Samples were placed randomly onto 384- well plates and were blinded for disease status. The design of the assay and the SNP genotyping of IL-17A rs2275913G>A and rs3748067C>T and IL-17F rs763780 T>C were performed by Bio Miao Biological Technology (Beijing, China) using the Sequenom MassARRAY platform (Sequenom, San Diego, CA, USA) according to the manufacturer instructions. The results of all duplicated samples were 100% consistent. -
A functional copy-number variation in MAPKAPK2 predicts risk and prognosis of lung cancer
BinLiu, LeiYang, BinfangHuang, MeiCheng, HuiWang, YinyanLi - Cell press - 10 August 2012
… We genotyped the g.CNV-30450 for all 4,789 subjects by using the TaqMan real-time quantitative PCR (qPCR) method according to the protocol of Applied BioSystems (catalog no. Hs01173160, Applied BioSystems, Foster City, CA, USA)11 and validated the results with the AccuCopy assay (a multiple competitive real-time PCR) by Genesky Bio-Tech (Shanghai, China) and Affymetrix Genome-Wide Human SNP Array 6.0 by Bio Miao Biological Technology (Beijing) in 200 randomly selected samples (Figures S1 and S2);12 the results were 97.5% or 98.0% concordant… -
MiR-509-3p enhances platinum drug sensitivity in ovarian cancer
LeileiNiu, HuaNi, YingziHou, QuanDu, HuaLi - Elsevier - 20 February 2019
… miRNA microarray system (Illumina) composed of probes targeting 2003 human miRNAs wasemployed to identify miRNA expression profile of platinum-sensitive and -resistant human ovariancancers by Biomiao (Biomiao, China) according to product protocol … -
Association of ENAM, TUFT1, MMP13, IL1B, IL10 and IL1RN gene polymorphism and dental caries sus
Xiao-Pan Hu, Tian-Zhu Song, Yan-Yan Zhu - Journal of International Medical Research - February 25, 2019
…Non-irritating saliva (2 ml) was collected from each child and added to saliva protection fluid (Olegene saliva DNA collector; Guangzhou Deep Blue Gene Technology, Guangzhou, China). The saliva samples were stored at –20C until processing. Genomic DNA was extracted from each saliva sample using a Genomic DNA Isolation Kit (BioMiao Biological Technology, Beijing, China) according to the manufacturer’s instructions. All of the seven SNPs in the six candidate genes were genotyped using iPLEX MassARRAYVR system (Agena Bioscience, San Diego, CA, USA), which -
TNF-a (-238G/A and -308G/A) gene polymorphisms may not contribute to the risk of ischemic stroke
Lian Gu, Guangliang Wu, Li Su, Yan Yan, Baoyun Liang, Jinjing Tan - International Journal of Neuroscience - Published online: 22 May 2015
… Genotyping of rs361525 and rs1800629 was per- formed on 384-well plates on the Sequenom MassAR- RAY platform (Sequenom, San Diego, CA, USA) [17] at the Center for Genotyping and Analysis of BIO MIAO Biological (Beijing, China) … -
Targeted P2X7R shRNA delivery attenuates sympathetic nerve sprouting and ameliorates cardiac dysfunct
Hongmei Gao ,Jie Yin, Yugen Shi, Hesheng Hu ,Xiaolu Li, Mei Xue ,Wenjuan Cheng - ORIGINAL RESEARCH ARTICLE - 31 December 2016
…(ii)sense:5′‐CUCUUGAGGAGCGCCGAAA‐3′; antisense: 5′‐UUUCGGCGCUCCUCAAGAG‐3′. The double‐strand oligonucleotides were ligated into the RNAi‐Ready pSIREN‐DNR‐DsRed‐Express Vector (Biomiao, Beijing, China). Then, the donor vector was inserted into the pLP‐Adeno‐X LP CMV Vector (Biomiao) to be constructed as the recombinant pLP‐Adeno‐X P2X7R shRNA (Ad‐sh P2X7R5a)…. -
Increased osteogenesis in osteoporotic bone marrow stromal cells by overexpression of leptin
Baoyu ZhengJun JiangKai Luo Lingling LiuMinkui LinYulin ChenFuhua Yan - Cell and Tissue Research - September 2015
… The cloned leptin fragment was ligated into a shuttle vector (pShuttle-CMV-EGFP;BioMiao Biological Technology, Beijing,China) to generate the recombined shuttle vector (pShuttle-hLEP-EGFP). The pShuttle-hLEP-
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Role of IL-17 gene polymorphisms in the susceptibility to gastric cancer
W.T. Qi , J.L. Gao and S.S. Zhang1 - Genetics and Molecular Research - October 26, 2015
Samples were placed randomly onto 384- well plates and were blinded for disease status. The design of the assay and the SNP genotyping of IL-17A rs2275913G>A and rs3748067C>T and IL-17F rs763780 T>C were performed by Bio Miao Biological Technology (Beijing, China) using the Sequenom MassARRAY platform (Sequenom, San Diego, CA, USA) according to the manufacturer instructions. The results of all duplicated samples were 100% consistent. -
A functional copy-number variation in MAPKAPK2 predicts risk and prognosis of lung cancer
BinLiu, LeiYang, BinfangHuang, MeiCheng, HuiWang, YinyanLi - Cell press - 10 August 2012
… We genotyped the g.CNV-30450 for all 4,789 subjects by using the TaqMan real-time quantitative PCR (qPCR) method according to the protocol of Applied BioSystems (catalog no. Hs01173160, Applied BioSystems, Foster City, CA, USA)11 and validated the results with the AccuCopy assay (a multiple competitive real-time PCR) by Genesky Bio-Tech (Shanghai, China) and Affymetrix Genome-Wide Human SNP Array 6.0 by Bio Miao Biological Technology (Beijing) in 200 randomly selected samples (Figures S1 and S2);12 the results were 97.5% or 98.0% concordant… -
MiR-509-3p enhances platinum drug sensitivity in ovarian cancer
LeileiNiu, HuaNi, YingziHou, QuanDu, HuaLi - Elsevier - 20 February 2019
… miRNA microarray system (Illumina) composed of probes targeting 2003 human miRNAs wasemployed to identify miRNA expression profile of platinum-sensitive and -resistant human ovariancancers by Biomiao (Biomiao, China) according to product protocol … -
Association of ENAM, TUFT1, MMP13, IL1B, IL10 and IL1RN gene polymorphism and dental caries sus
Xiao-Pan Hu, Tian-Zhu Song, Yan-Yan Zhu - Journal of International Medical Research - February 25, 2019
…Non-irritating saliva (2 ml) was collected from each child and added to saliva protection fluid (Olegene saliva DNA collector; Guangzhou Deep Blue Gene Technology, Guangzhou, China). The saliva samples were stored at –20C until processing. Genomic DNA was extracted from each saliva sample using a Genomic DNA Isolation Kit (BioMiao Biological Technology, Beijing, China) according to the manufacturer’s instructions. All of the seven SNPs in the six candidate genes were genotyped using iPLEX MassARRAYVR system (Agena Bioscience, San Diego, CA, USA), which -
TNF-a (-238G/A and -308G/A) gene polymorphisms may not contribute to the risk of ischemic stroke
Lian Gu, Guangliang Wu, Li Su, Yan Yan, Baoyun Liang, Jinjing Tan - International Journal of Neuroscience - Published online: 22 May 2015
… Genotyping of rs361525 and rs1800629 was per- formed on 384-well plates on the Sequenom MassAR- RAY platform (Sequenom, San Diego, CA, USA) [17] at the Center for Genotyping and Analysis of BIO MIAO Biological (Beijing, China) … -
Targeted P2X7R shRNA delivery attenuates sympathetic nerve sprouting and ameliorates cardiac dysfunct
Hongmei Gao ,Jie Yin, Yugen Shi, Hesheng Hu ,Xiaolu Li, Mei Xue ,Wenjuan Cheng - ORIGINAL RESEARCH ARTICLE - 31 December 2016
…(ii)sense:5′‐CUCUUGAGGAGCGCCGAAA‐3′; antisense: 5′‐UUUCGGCGCUCCUCAAGAG‐3′. The double‐strand oligonucleotides were ligated into the RNAi‐Ready pSIREN‐DNR‐DsRed‐Express Vector (Biomiao, Beijing, China). Then, the donor vector was inserted into the pLP‐Adeno‐X LP CMV Vector (Biomiao) to be constructed as the recombinant pLP‐Adeno‐X P2X7R shRNA (Ad‐sh P2X7R5a)…. -
Increased osteogenesis in osteoporotic bone marrow stromal cells by overexpression of leptin
Baoyu ZhengJun JiangKai Luo Lingling LiuMinkui LinYulin ChenFuhua Yan - Cell and Tissue Research - September 2015
… The cloned leptin fragment was ligated into a shuttle vector (pShuttle-CMV-EGFP;BioMiao Biological Technology, Beijing,China) to generate the recombined shuttle vector (pShuttle-hLEP-EGFP). The pShuttle-hLEP-